Inhibition by glucocorticoids of tumor necrosis factor‐mediated cytotoxicity

FEBS Letters

Fiers, Walter¹  Beyaert, Rudi¹  Roy, Frans Van¹  Suffys, Philip¹ 

[1] Laboratory of Molecular Biology, State University of Gent, 9000 Gent, Belgium

关键词: Tumor necrosis factor;    Lipocortin;    Phospholipase;    Dexamethasone;    TNF;    tumor necrosis factor;    PLA2;    phospholipase A2;    DEX;    dexamethasone;    AA;    arachidonic acid;    EGF;    epidermal growth factor;   

DOI  :  10.1016/0014-5793(90)80161-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The role of the phospholipase inhibitor proteins, lipocortin-I and -II, in tumor necrosis factor (TNF)-mediated cytotoxicity against L929 fibrosarcoma cells was investigated. We previously reported that TNF-mediated cytotoxicity was inhibited by dexamethasone (DEX), suggesting an involvement of lipocortins [1]. Now we show that, despite inhibition by DEX of TNF-induced arachidonic acid release, DEX has no effect on the synthesis of these lipocortins. Moreover, TNF itself has no effect on the synthesis and phosphorylation of lipocortin-I and -II. Also there was no difference in expression levels of lipocortin-I and -II between TNF-sensitive and -resistant cells. These data strongly suggest that the protective effect of DEX and other glucocorticoids is not mediated by lipocortins.

【 授权许可】

Unknown