Monoclonal antibodies to lipocortin‐1 as probes for biological function

FEBS Letters

Sinclair, Lesley K.¹  Lawton, Pornsri¹  Dougas, Irene¹  Liang, Chi-Ming¹  Pepinsky, R.Blake¹  Browning, Jeffrey L.¹ 

[1] Biogen Inc., 14 Cambridge Center, Cambridge, MA 02142, USA

关键词: Lipocortin;    Annexin;    Phospholipid binding protein;    Ca2+ dependent;   

DOI  :  10.1016/0014-5793(90)80564-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have developed two monoclonal antibodies to human lipocortin-1 (103 and 105) as reagents for quantitating the protein in biological systems and neutralizing its activity. Lipo 105 is a high affinity antibody that is functional in ELISA and Western blot formats. The antibody recognizes a site between amino acids 30 and 55 in the lipocortin-1 sequence and can be used on native or denatured protein. Lipo 103 is an antibody that neutralizes the phospholipase A₂ inhibitory activity of lipocortin-1 by blocking binding of the protein to phospholipid surfaces. The antibody is specific for native human lipocortin-1. Lipo 103 was recently shown to block lipocortin-1-dependent differentiation of a squamous carcinoma cell line, demonstrating its usefulness as a probe for function.

【 授权许可】

Unknown