Tumor necrosis factor and interleukin 1 activate phospholipase in rat chondrocytes

FEBS Letters

Fiers, Walter¹  Van Roy, Frans¹  Suffys, Philip¹ 

[1] Laboratory of Molecular Biology, State University of Ghent, 9000 Ghent, Belgium

关键词: Tumor necrosis factor;    Interleukin 1;    Phospholipase;    (Rat chondrocyte);    IL-1;    interleukin 1;    PGE2;    prostaglandin E2;    PLA2;    phospholipase A2;    TNF;    tumor necrosis factor;   

DOI  :  10.1016/0014-5793(88)80379-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tumor necrosis factor (TNF) and interleukin 1 (IL-1) are both cytokines of macrophage origin with similar activity on several cell types. We investigated whether TNF can, analogously to IL-1, stimulate phospholipase activity of chondrocytes. Addition of each of these cytokines to cells, isolated from the xiphisternum of adult rats, resulted in a time- and dose-dependent increase in phospholipase activity in both secreted and membrane-associated form. Moreover, TNF and IL-1 both induce a transformation of chondrocyte morphology. In conclusion, TNF stimulates chondrocyte phospholipase activity and extends the long list of actions shared by IL-1 and TNF in a diversity of cellular systems.

【 授权许可】

Unknown