P‐glycoproteins encoded by mdr 1b in murine gravid uterus and multidrug resistant tumor cell lines are differentially glycosylated

FEBS Letters

Horwitz, Susan Band¹  Greenberger, Lee M.¹  Croop, James M.²  Arceci, Robert J.² 

[1] Departments of Molecular Pharmacology and Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461 USA;Pediatric Hematology/Oncology, Dana-Farber Cancer Institute and The Children's Hospital, Boston, MA 02115, USA

关键词: Multidrug resistance;    P-glycoprotein;    Glycosylation;    Pregnancy;    Chemotherapy;    Mouse endometrium;   

DOI  :  10.1016/0014-5793(89)81586-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

There are 3 members of the multidrug-resitance gene family expressed in mouse. Only one of these, mdr lb, and its gene product P-glycoprotein are induced to high levels in the mouse endometrium during pregnancy. It is shown here that P-glycoprotein in the gravid uterus is significantly larger (M _r 155000) compared to P-glycoprotein encoded by mdr lb in a murine multidrug-resistant cell line (M _r 140000). However, both species co-migrate after enzymatic removal of N-linked sugars (M _r 125000). These results demonstrate that differential glycosylation of the mdr lb gene product contributes to molecular heterogeneity found in P-glycoprotein from normal and multidrug-resistant cells.

【 授权许可】

Unknown