Primary structures of new ‘iso‐hirudins’

FEBS Letters

Engels, J.²  Scharf, M.²  Tripier, D.¹ 

[1] Hoechst A G, Frankfurt a. Main, FRG;Institut für organische Chemie, Johann Wolfgang v. Goethe Universität, Niederurseler Hang, D-6000 Frankfurt 50 FRG

关键词: Hirudin;    Microsequencing;    N-terminal;    HPLC;    microbore;    Cleavage;    Asn-Gly;    Trypsin digestion;    HPLC;    high-performance liquid chromatography;    TPCK;    N-tosyl-L-phenylalanine chloromethyl ketone;    TFA;    trifluoroacetic acid;    PTH-amino acid;    phenylthiohydantoin amino acid;   

DOI  :  10.1016/0014-5793(89)81070-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Crude hirudin (12.7 U/μg), a complex mixture of polypeptides obtained from the leech, could be separated by microbore HPLC. A combination of amino acid analysis, N-terminal microsequencing and chemical as well as enzymatic fragmentation made the primary sequence of the new isohirudins Ia-IIIb' accessible. The biological activity determined in the thrombin inhibition test showed a comparable value for all of these compounds. The results presented address the question as to whether these isohirudins are true mutations from a family of genes or a family of leeches.

【 授权许可】

Unknown