Amino acid sequence of pheromone‐inducible surface protein in Enterococcus faecalis, that is encoded on the conjugative plasmid pPD1

FEBS Letters

Nagasawa, Hiromich¹  Nakayama, Jiro¹  Clewell, Don B.²  Isogai, Akira¹  Suzuki, Akinori¹ 

[1] Department of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan;Departments of Oral Biology and Microbiology/Immunology, School of Dentistry and Medicine, The University of Michigan, Ann Arbor, MI 48109, USA

关键词: Sex pheromone;    Conjugative plasmid;    Plasmid transfer;    Adhesin;    Aggregation substance;    Enterococcus faecalis;    SDS;    sodium dodecyl sulfate;    HPLC;    high-performance liquid chromatography;    kDa;    kilodalton;    kbp;    kilobase pair;    N-terminal;    amino terminal;    C-terminal;    carboxyl terminal;   

DOI  :  10.1016/0014-5793(90)80293-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The major pheromone-inducible protein, PD78, believed to contribute to bacterial conjugation, was purified from Enterococcus (formerly Streptococcus) faecalis cells containing the plasmid pPD1. A cloned EcoRI-Bg/II 3.6-kbp fragment of the plasmid pAM351 (pPD1::Tn916) contained an open reading frame corresponding to 467 amino acid residues representing PD78. In a central region of the deduced protein, there is a repeated sequence of X-X-Pro that is repeated 15 times. This is analogous to the Gln-Gln-Pro repeat in the C-terminal region of TraD product encoded on the R 100 plasmid in Escherichia coli.

【 授权许可】

Unknown