Correlation between fibronectin binding protein A expression level at the surface of recombinant lactococcus lactis and plasmid transfer in vitro and in vivo

BMC Microbiology

Jean-Marc Chatel³  Jean-Guy LeBlanc²  Philippe Langella³  Rebeca Martin³  Silvina del Carmen²  Alejandra de Moreno de LeBlanc²  Anderson Miyoshi¹  Vasco Azevedo¹  Denis Mariat³  Juliana F Almeida³ 

[1] Institute of Biological Sciences, Federal University of Minas Gerais (UFMG-ICB), Belo Horizonte, MG, Brazil;CERELA-CONICET, Chacabuco 145, San Miguel de Tucumán, Argentina;AgroParisTech, UMR1319 Micalis, Jouy-en-Josas, F-78350, France

关键词: Plasmid transfer;    Invasiveness;    BMDCs;    Caco-2 cells;    nisA promoter;    Lactococcus lactis;    FnBPA;   

Others  :  1170528
DOI  :  10.1186/s12866-014-0248-9

 received in 2014-07-08, accepted in 2014-09-17,  发布年份 2014

【 摘 要 】

Background

Fibronectin Binding Protein A (FnBPA) is an invasin from Staphylococcus aureus that allows this pathogen to internalize into eukaryote cells. It was previously demonstrated that recombinant Lactococcus lactis expressing FnBPA were invasive and able to transfer a plasmid to eukaryotic cells in vitro and in vivo. In this study, the invasivity of recombinant strains of Lactococcus lactis that express FnBPA under the control of its constitutive promoter or driven by the strong nisin inducible expression system (NICE) were studied.

Results

It was demonstrated that the nisA promoter allows an increase of FnBPA expression on the surface of Lactococcus lactis surface, as shown by flow cytometry, which subsequently enhanced internalization and plasmid transfer properties in vitro in Caco2 cells and Bone Marrow Dendritic Cells. In vivo, the use of nisA promoter increase the plasmid transfer in cells of both the small and large intestine of mice.

Conclusion

FnBPA expression at the surface of recombinant L. lactis is positively correlated to internalization and DNA transfer properties. The recombinant strains of L. lactis that expresses FnBPA under the control of the nisin inducible expression system could thus be considered as an improved tool in the field of DNA transfer.

【 授权许可】

   
2014 Almeida et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

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