N‐terminal amino acid sequence of the Rieske iron‐sulfur protein from the cytochrome b 6/f‐complex of spinach thylakoids

FEBS Letters

Meyer, Helmut E.¹  Pfefferkorn, Bernhard² 

[1] Institut für Pysiologische Chemie, Ruhr-Uiversität, Postfach 10 21 48, D-4630 Bochum 1, FRG;Lehrstuhl Biochemie der Pflanzen Ruhr-Uiversität, Postfach 10 21 48, D-4630 Bochum 1, FRG

关键词: Chloroplast;    Cytochrome;    b6/f-complex;    FeS protein;    Amino acid sequence;    PAGE;    polyacrylamide gel electrophoresis;    HPLC;    high performance liquid chromatography;    PTH;    phenylthiohydantoin;    PTC;    phenylthiocarbamyl;    N-terminal;    amino-terminal;    Mes;    2-(N-morpholino)ethanesulfonic acid;    kDa;    kilodalton;    CNBr;    cyanogen bromide;   

DOI  :  10.1016/0014-5793(86)80987-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The 20 kDa subunit of the cytochrome b ₆/f-complex, the Rieske iron-sulfur protein, was isolated by preparative SDS-PAGE. For automatic Edman degradation the protein was recovered by electrophoretic elution. Several peptides were purified after tryptic, CNBr or Arg C endopeptidase digestion or cleavage at Trp-residues. The determination of the amino acid sequence of these peptides yielded overlapping sequences for a total of 96 amino acids starting at the N-terminus. This sequence shows no homology to the primary structure of the two Rieske FeS proteins already published by other authors. But like these it contains a hydrophobic sequence of 30 amino acids close to the N-terminal indicating the sequence that spans the membrane. Two polypeptides, closer to the C-terminus, contained sequences which are also present in Neurospora crassa and Rhodopseudomonas sphaeroides including a Cys-Pro-Cys containing sequence involved in iron binding.

【 授权许可】

Unknown