Functional analysis of chloroplast early light inducible proteins (ELIPs)

Wetzel, Carolyn M

Smith College

关键词: Chloroplast;    Gene Expression;    Light Stress;    Thylakoid;    59 Basic Biological Sciences Chloroplast;   

DOI  :  10.2172/836992
RP-ID  :  Final Report
RP-ID  :  FG02-02ER15333
RP-ID  :  836992

美国|英语

来源: UNT Digital Library

【 摘 要 】

The objectives of this project were to characterize gene expression patterns of early light inducible protein (ELIP) genes in Arabidopsis thaliana and in Lycopersicon esculentum, to identify knock mutants of the 2 ELIP genes in Arabidopsis, and to characterize the effects of the knockouts. Expression in Arabidopsis was studied in response to thylakoid electron transport chain (PETC) capacity, where it was found that there is a signal for expression associated with reduction of the PETC. Expression in response to salt was also studied, with different responses of the two gene copies. Knockout lines for ELIP1 and ELIP2 have been identified and are being characterized. In tomato, it was found that the single-copy ELIP gene is highly expressed in ripening fruit during the chloroplast-to-chromoplast transition. Studies of expression in tomato ripening mutants are ongoing.