Human brain fibroblast growth factor

FEBS Letters

Esch, Frederick¹  Böhlen, Peter¹  Jones, Kenneth L.¹  Baird, Andrew¹  Gospodarowicz, Denis¹ 

[1] Laboratories for Neuroendocrinology, The Salk Institute for Biological Studies, La Jolla, CA 92138 U.S.A.

关键词: Heparin-Sepharose affinity chromatography;    HPLC;    Microsequencing;    Endothelial cell proliferation;    Radioimmunoassay;   

DOI  :  10.1016/0014-5793(85)80765-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Fibroblast growth factor (FGF) has been purified to homogeneity from human brain by a procedure involving salt precipitation, cation-exchange chromatography, Heparin-Sepharose affinity chromatography and reverse-phase HPLC. Isolation was monitored by radioimmunoassay and/or by testing column fractions for their capacity to stimulate the proliferation of vascular endothelial cells in vitro. The amino-terminal sequence of human brain FGF was, determined as Pro-Ala-Leu-Pro-Glu-Asp-Gly-Gly-Ser-Gly-Ala-PhePro-. This sequence is identical to that of the amino-terminal region of bovine FGF. Additional evidence, including amino acid composition, Chromatographie retention behavior, and immunoreactivity suggest that the human and bovine mitogens are very similarin structure.

【 授权许可】

Unknown