Partial molecular characterization of endothelial cell mitogens from human brain: acidic and basic fibroblast growth factors

FEBS Letters

Böhlen, Peter¹  Fràter-Schröder, Marijke¹  Gautschi, Peter¹ 

[1] Biochemisches Institut, Universität Zürich, Winterthurerstr. 190, CH-8057 Zürich, Switzerland

关键词: Fibroblast growth factor Heparin-Sepharose affinity chromatography HPLC Microsequencing Microheterogeneity;   

DOI  :  10.1016/0014-5793(86)80812-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two endothelial cell growth factors have been isolated from human brain extracts by a procedure involving heparin-Sepharose affinity chromatography and reverse-phase HPLC. On the basis of molecular size, amino acid composition and extended amino-terminal sequences, the two proteins are structurally closely related to bovine acidic and basic fibroblast growth factors (aFGF, bFGF). The N-terminal sequences of human and bovine aFGF diner in two positions while those for bFGF are indistinguishable. Human aFGF is microheterogenous: in addition to aFGF two N-terminally truncated forms lacking the first and the seven first amino acids, respectively, were characterized. Human and bovine mitogens possess similar mitogenic activities.

【 授权许可】

Unknown