FEBS Letters | |
Heterogeneity of native rat liver elongation factor 2 | |
Marzouki, Abdelkader1  Reboud, Anne-Marie1  Reboud, Jean-Paul1  Lavergne, Jean-Pierre1  | |
[1] Laboratoire de Biochimie Médicale, Université Lyon 1, UM CNRS 24, 43, Boulevard du 11 Novembre 1918, 69622-Villeurbanne, France | |
关键词: Elongation factor 2; ADP-ribosylation; Phosphorylation; (Rat liver); | |
DOI : 10.1016/0014-5793(89)81063-4 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The high heterogeneity of native rat liver EF-2 prepared from either 105000 x g supernatant or microsome high-salt extract was detected by two-dimensional equilibrium isoelectric focusing-SDS-polyacrylamide gel electrophoresis in the presence of 9.5 M urea. Five spots were always detected, all of M r 95000, which were not artefactual for their amount varied when EF-2 was specifically ADP-ribosylated by diphtheria toxin in the presence of NAD+, and/or phosphorylated on a threonine residue by a Ca2+/calmodulin-dependent protein kinase (most likely Ca2+/calmodulin-dependent protein kinase III described by others [(1987) J. Biol. Chem. 262, 17299–17303; (1988) Nature 334, 170–173]). Results of ADP-ribosylation and/or phosphorylation experiments with either unlabeled or labeled reagents ([14C]NAD and [32P]ATP) strongly suggest that our preparation contained native ADP-ribosylated and native phosphorylated forms which could be estimated at about 20% and 40% of the whole EF-2. Phosphorylated and ADP-ribosylated forms of EF-2 could be ADP-ribosylated and phosphorylated, respectively, but a native form both ADP-ribosylated and phosphorylated was not detected. Our results also suggest the existence of a minor native form of EF-2 and of its phosphorylated and ADP-ribosylated derivatives.
【 授权许可】
Unknown
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