ADP‐ribosylated elongation factor 2 (ADP‐ribosyl‐EF‐2) is unable to promote translocation within the ribosome

FEBS Letters

Ovchinnikov, L.P.¹  Davydova, E.K.¹ 

[1] Institute of Protein Research, Academy of Sciences of the USSR, Pushchino, Moscow Region, USSR

关键词: Elongation factor 2;    ADP-ribosylation;    Diphtheria toxin;    Elongation factor 2-promoted translocation;    EF;    elongation factor;    ADP-ribosyl-EF-2;    ADP-ribosylated EF-2;    GMPPCP;    guanosine 5'-(β;    γ-methylene)triphosphate;    NAD;    nicotinamide adenine dinucleotide;    poly(U);    polyuridylic acid;   

DOI  :  10.1016/0014-5793(90)80589-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Elongation factor 2 (EF-2), ADP-ribosylated in vitro by the A-fragment of diphtheria toxin, can (in the presence of GMPPCP) form stable corn plexes with ribosomes regardless of whether the ribosomes are empty or carrying poly(U) and Phe-tRNA in the A-site. Despite its efficient binding to ribosomes, ADP-ribosyl-EF-2, in contrast to the non-modified EF-2 is unable to promote the shift of Phe-tRNA from the A-site to the P-sit of the ribosome as determined by the puromycin reaction, i.e. it is incapable of promoting the translocation reaction within the ribosome.

【 授权许可】

Unknown