Endogenous ADP‐ribosylation of elongation factor 2 in polyribosome fraction of rabbit reticulocytes

FEBS Letters

Ovchinnikov, L.P.¹  Davydova, E.K.¹  Sitikov, A.S.¹ 

[1] Institute of Protein Research, Academy of Sciences of the USSR, 142292 Pushchino, Moscow Region, USSR

关键词: ADP-ribosylation;    Elongation factor 2;    Polyribosome;    Rabbit reticulocyte;    Protein biosynthesis regulation;   

DOI  :  10.1016/0014-5793(84)80953-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Several polypeptides of about 120, 96, 85, 60 and 38 kDa are shown to be radiolabeled during incubation of the mono- and polyribosome fraction of rabbit reticulocytes with [³²P]NAD. Among them is a polypeptide coinciding with elongation factor 2 (EF-2) in its electrophoretic mobility in SDS-polyacrylamide gel. The addition of pure EF-2 to the polyribosome fraction results in an increase of the radioactive label in this polypeptide band. From this it is concluded that both endogenous and added EF-2 is ADP-ribosylated by an enzyme associated with polyribosomes. A possibility of regulation of protein synthesis through endogenous ADP-ribosylation in vivo is considered.

【 授权许可】

Unknown