Evidence for the existence of a cardiac specific isoform of the α1 subunit of the voltage dependent calcium channel

FEBS Letters

Engle, Dorothy B.¹  Varadi, Gyula¹  Dascal, Nathan²  Singer, Datna²  Slish, Donald F.¹  Schwartz, Arnold¹  Lotan, Ilana² 

[1] Department of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, 231 Bethesda Avenue, Cincinnati, OH 45267-0575, USA;Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv 69978, Israel

关键词: Ca2+ channel;    voltage-dependent;    Dihydropyridine receptor;    Ion channel;    cDNA cloning;    VDCC;    voltage-dependent calcium channel;    DHP;    1;    4-dihydropyridine;   

DOI  :  10.1016/0014-5793(89)80786-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Biochemical, pharmacological and electrophysiological evidence implies the existence of tissue specific isoforms of the L-type VDCC. The α₁ and α₂ subunits of the skeletal muscle calcium channel have been previously cloned and their amino acid sequence deduced. Here we report the isolation and sequencing of a partial cDNA that encodes a heart specific isoform of the α₁ subunit. The amino acid sequence deduced from this part cDNA clone shows 64.7% similarity with the skeletal muscle α₁ subunit. Northern analysis reveals 2 hybridizing bands, 8.5 and 13 kb, in contrast to one 6.5 kb band in the skeletal muscle. Selective inhibition of mRNA expression in Xenopus oocytes by complementary oligodeoxynucleotides derived from the heart clone provides further evidence that the cDNA corresponds to an essential component of the VDCC. These data further support the existence of tissue-specific isoforms of the L-type VDCC.

【 授权许可】

Unknown