Monoclonal antibodies against the 1,4‐dihydropyridine receptor associated with voltage‐sensitive Ca2+ channels detect similar polypeptides from a variety of tissues and species

FEBS Letters

Norman, R.I.¹  Burgess, A.J.¹  Harrison, T.M.²  Allen, E.² 

[1] Department of Medicine, Clinical Sciences Building, Leicester Royal Infirmary, PO Box 65, Leicester LE2 7LX, England;Department of Biochemistry, University of Leicester, University Road, Leicester LEI 7RH, England

关键词: Dihydropyridine;    Dihydropyridine receptor;    Ca2+ channel;    Monoclonal antibody;   

DOI  :  10.1016/0014-5793(87)81570-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Four monoclonal antibodies have been raised against voltage-sensitive Ca²⁺ channel dihydropyridine receptors from rabbit skeletal muscle. When tested by immunoblot assay of denatured transverse tubule membranes in reducing polyacrylamide gels, each recognised a single polypeptide of M_r ~ 140000 that co-migrated with the large glycoprotein subunit of the purified receptor. In blots of nonreducing gels, a larger protein of M_r ~ 170000 was seen and three of the antibodies recognised additional components at M_r ~ 310000 and ~ 330000. Crossreactive material of similar molecular mass was also seen in rabbit heart and brain, and in the skeletal muscle of other species.

【 授权许可】

Unknown