Developmental regulation of expression of the α1 and α2 subunits mRNAs of the voltage‐dependent calcium channel in a differentiating myogenic cell line

FEBS Letters

Orlowski, John¹  Varadi, Gyula²  Schwartz, Arnold² 

[1] Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, 231 Bethesda Avenue, Cincinnati, OH 45267-0575, USA;Department of Pharmacology and Cell Biophysics University of Cincinnati College of Medicine, 231 Bethesda Avenue, Cincinnati, OH 45267-0575, USA

关键词: Ca2+ channel;    voltage-dependent;    mRNA;    Developmental expression;    Subunit;    α2;    Subunit;    α1;    (C2Cl2 myogenic cell);    PDGF;    platelet-derived growth factor;    EGF;    epidermal growth factor;    FGF;    fibroblast growth factor;    TGF-β;    tumor growth factor-β;    ACh;    acetylcholine;    VDCC;    voltage-dependent calcium channel;    SSPE;    150 mM NaCl;    10 mM NaH2PO4;    1 mM EDTA;    pH 7.4;   

DOI  :  10.1016/0014-5793(89)80787-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The voltage-dependent calcium channel (VDCC) in skeletal muscle probably plays a key role in transducing membrane charge movement to the calcium release channel. We report here that the expression of VDCC α₁ and α₂ mRNAs is developmentally regulated in differentiating C2Cl2 myogenic cells. The α₁ mRNA is not detectable in the myoblast form of C2Cl2 cells while its expression is induced 20-fold in differentiated myotubes. In contrast, the α₂ mRNA is weakly expressed in myoblasts but is also induced upon myogenic differentiation.

【 授权许可】

Unknown