The constituents of storage granules in the dermal glands of Xenopus laevis Structure of a basic polypeptide deduced from cloned cDNA

FEBS Letters

Kreil, Günther¹  Berger, Hans¹ 

[1] Molecular Biology Institute, Austrian Academy of Sciences, A-5020 Salzburg, Austria

关键词: cDNA cloning;    Nucleotide sequence;    Amino acid sequence;    (Amphibian skin;    Secretory gland);    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(89)80644-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mature dermal glands of Xenopus laevis contain storage granules with a characteristic ellipsoid shape. A few major proteins are present in these granules, including two with the same amino-terminal sequence and apparent molecular masses of 26 and 28 kDa. Using antibodies against these proteins, positive clones were isolated from a cDNA expression library prepared from skin of X. laevis. One cDNA encodes a preprotein with a typical signal sequence and a mature part of 187 amino acids. The protein shows internal homology at both the amino and carboxyl end. The latter part has a very high content of basic amino acids.

【 授权许可】

Unknown