FEBS Letters | |
The constituents of storage granules in the dermal glands of Xenopus laevis Structure of a basic polypeptide deduced from cloned cDNA | |
Kreil, Günther1  Berger, Hans1  | |
[1] Molecular Biology Institute, Austrian Academy of Sciences, A-5020 Salzburg, Austria | |
关键词: cDNA cloning; Nucleotide sequence; Amino acid sequence; (Amphibian skin; Secretory gland); SDS-PAGE; SDS-polyacrylamide gel electrophoresis; | |
DOI : 10.1016/0014-5793(89)80644-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Mature dermal glands of Xenopus laevis contain storage granules with a characteristic ellipsoid shape. A few major proteins are present in these granules, including two with the same amino-terminal sequence and apparent molecular masses of 26 and 28 kDa. Using antibodies against these proteins, positive clones were isolated from a cDNA expression library prepared from skin of X. laevis. One cDNA encodes a preprotein with a typical signal sequence and a mature part of 187 amino acids. The protein shows internal homology at both the amino and carboxyl end. The latter part has a very high content of basic amino acids.
【 授权许可】
Unknown
【 预 览 】
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