Molecular cloning and sequencing of human cDNA for phosphoribosyl pyrophosphate synthetase subunit II

FEBS Letters

Ishijima, Sumio¹  Shimada, Hideaki¹  Taira, Masanori¹  Tatibana, Masamiti¹  Iizasa, Taizo¹ 

[1] Department of Biochemistry, Chiba University School of Medicine, Inohana, Chiba 280, Japan

关键词: Ribose-phosphate pyrophosphokinase;    Enzyme subunit;    cDNA cloning;    Nucleotide sequence;    Amino acid sequence;    (Human);    PPRibP;    5-phosphoribosyl 1-pyrophosphate;    PRS I (II);    phosphoribosyl pyrophosphate synthetase subunit I (II);    kb;    kilbase(s);    bp;    base pairs;    dT;    deoxythymidylic acid;    poly(A);    polyadenylic acid;   

DOI  :  10.1016/0014-5793(89)81159-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

cDNA clones for human phosphoribosyl pyrophosphate synthetase subunit II (PRS II) were isolated. The five overlapping clones contained 2457 base pairs (bp) covering a 954-bp complete coding region for 318 amino acid residues. Homologies between human and rats PRS II were 99% of the amino acid and 88% of the nucleotides in the coding region. This amino acid homology seems to be the highest so far reported for enzymes involved in nucleotide metabolism and glycolysis. The highly conserved structure may be required for unique catalysis and rigid regulation of this enzyme.

【 授权许可】

Unknown