Human cone‐specific cGMP phosphodiesterase α′ subunit: Complete cDNA sequence and gene arrangement

FEBS Letters

Andreeva, Svetlana G.²  Zagranichny, Vasily E.¹  Smirnova, Evgeniya V.²  Feshchenko, Elena A.²  Suslova, Veronika A.²  Lipkin, Valery M.² 

[1] Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 142292 Pushchino, Moscow Region, Russia;Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya St. 16/10, 117871 GSP Moscow V-437, Russia

关键词: Cyclic GMP phosphodiesterase (PDE);    cDNA cloning;    Human retina;    Nucleotide sequence;    CAAX;    Cys-aliphatic-aliphatic-X;   

DOI  :  10.1016/0014-5793(96)00104-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Four independent phage clones containing the fragments of cone-specific cGMP phosphodiesterase (PDE) α′ subunit (PDEα′) cDNA were isolated from the human cDNA library. The screening of the genomic library resulted in isolation of four independent phage clones with the fragments of human cone PDEα′ gene including 5′-flanling region and exons ranged from 1 to 14 (overall 32 kilobases). Structural studies of the clones made it possible to establish the complete human cone PDEα′ cDNA structure (3455 base pairs). The encoding polypeptide consists of 858 amino acid residues with a calculated molecular mass of 99169 Da. The deduced amino acid sequence displays high homology to the earlier analyzed catalytic α, β and α′ subunits of bovine, human, chicken and mouse photoreceptor PDEs.

【 授权许可】

Unknown