Nucleotide sequence and organization of potato leafroll virus genomic RNA

FEBS Letters

Huisman, Marianne J.²  Goldbach, Rob³  Cornelissen, Ben J.C.²  Huttinga, Harm¹  van der Wilk, Frank³ 

[1] MOGEN International NV, Einsteinweg 97, 2333 CB Leiden, The Netherlands;Agricultural University Wageningen, Department of Virology, PO Box 8045, 6700 EM Wageningen, The Netherlands;Research Institute for Plant Protection, PO Box 9060, 6700 GW Wageningen The Netherland

关键词: cDNA cloning;    Nucleotide sequence;    Amino acid sequence homology;    RNA virus;    plant positive-strand;    (Potato leafroll virus);    BWYV;    beet western yellows virus;    BYDV;    barley yellow dwarf virus;    CarMV;    carnation mottle virus;    CPMV;    cowpea mosaic virus;    ORF;    open reading frame;    PLRV;    potato leafroll virus;   

DOI  :  10.1016/0014-5793(89)80190-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The nucleotide sequence of the genomic RNA of potato leafroll virus was determined and its genetic organization deduced. The RNA is 5882 nucleotides long and contains 6 open reading frames (ORFs) encoding proteins of 70, 70, 56, 28, 23 and 17 kDa. The putative genes for the coat protein (23 kDa) and the RNA-dependent RNA polymerase (70 kDa) were identified by interviral amino acid sequence homologies. For expression of the different ORFS, translational frameshift and readthrough events are proposed.

【 授权许可】

Unknown