FEBS Letters | |
Isolation and sequence analysis of a cDNA clone encoding a type‐1 protein phosphatase catalytic subunit: Homology with protein phosphatase 2A | |
da Cruz e Silva, Edgar F.1  Caudwell, F.Barry1  da Cruz e Silva, Odete B.1  Berndt, Norbert1  Cohen, Patricia T.W.1  Campbell, David G.1  Cohen, Philip1  | |
[1] Department of Biochemistry, University of Dundee, Dundee DD1 4HN, Scotland | |
关键词: Protein phosphatase; cDNA cloning; Nucleotide sequence; Amino acid sequence homology; | |
DOI : 10.1016/0014-5793(87)80316-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
A 1.5 kb clone containing the full-length coding sequence of a type-1 protein phosphatase catalytic subunit has been isolated from a rabbit skeletal muscle cDNA library constructed in λgt10. The protein sequence deduced from the cDNA contains 311 residues and has a molecular mass of 35.4 kDa. A single mRNA species at 1.6 kb was visualized by Northern blotting. The type-1 protein phosphatase was strikingly homologous to protein phosphatase 2A, 49% of the amino acids between residues 11 and 280 being identical. The first 10 and last 31 residues were dissimilar. Residues 1–101 of the type-1 protein phosphatase also showed 21% sequence identity with a region of mammalian alkaline phosphatases.
【 授权许可】
Unknown
【 预 览 】
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