Isolation and sequence analysis of a cDNA clone encoding a type‐1 protein phosphatase catalytic subunit: Homology with protein phosphatase 2A

FEBS Letters

da Cruz e Silva, Edgar F.¹  Caudwell, F.Barry¹  da Cruz e Silva, Odete B.¹  Berndt, Norbert¹  Cohen, Patricia T.W.¹  Campbell, David G.¹  Cohen, Philip¹ 

[1] Department of Biochemistry, University of Dundee, Dundee DD1 4HN, Scotland

关键词: Protein phosphatase;    cDNA cloning;    Nucleotide sequence;    Amino acid sequence homology;   

DOI  :  10.1016/0014-5793(87)80316-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A 1.5 kb clone containing the full-length coding sequence of a type-1 protein phosphatase catalytic subunit has been isolated from a rabbit skeletal muscle cDNA library constructed in λgt10. The protein sequence deduced from the cDNA contains 311 residues and has a molecular mass of 35.4 kDa. A single mRNA species at 1.6 kb was visualized by Northern blotting. The type-1 protein phosphatase was strikingly homologous to protein phosphatase 2A, 49% of the amino acids between residues 11 and 280 being identical. The first 10 and last 31 residues were dissimilar. Residues 1–101 of the type-1 protein phosphatase also showed 21% sequence identity with a region of mammalian alkaline phosphatases.

【 授权许可】

Unknown