The human rod photoreceptor cGMP phosphodiesterase β‐subunit

FEBS Letters

Terpugov, B.E.¹  Atabekova, N.V.¹  Lipkin, V.M.¹  Shmukler, B.E.¹  Feshchenko, E.A.¹  Rakitina, T.V.¹  Khramtsov, N.V.¹  Suslova, V.A.¹ 

[1] Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Muklaya 16110, 117871 GSP Moscow V-437, Russian Federation

关键词: Cyclic GMP phosphodiesterase;    cDNA cloning;    Polymerase chain reaction;    Alternative splicing;    Human retina;    CAAX;    Cys-aliphatic-aliphatic-X;   

DOI  :  10.1016/0014-5793(93)81003-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

cDNA clones encoding the β-subunit of the photoreceptor cGMP phosphodiesterase (PDE) were isolated from a human retina library and their sequence was determined. The encoded polypeptide consists of 854 amino acid residues with a calculated molecular mass of 98,416 Da. Alignment of the deduced amino acid sequence with the earlier analysed α-, β- and α'-subunits of bovine and mouse PDEs demonstrates a high homology. Two overlapping recombinant λ phage clones containing 26 kb of the human PDE β-subunit gene were isolated from the genomic library. A total nucleotide sequence of exons 4–22 of the PDE β-subunit gene was established which completely corresponded to the cDNA structure. According to sequence analysis no potential possibility for alternative splicing of the β-subunit gene was observed between exons 20 and 21 which led to the formation of the β'-subunit as described for mouse PDE. Polymerase chain reaction (PCR) experiments also confirm the absence of the PDE β'-subunit in human retina.

【 授权许可】

Unknown