FEBS Letters | |
The human rod photoreceptor cGMP phosphodiesterase β‐subunit | |
Terpugov, B.E.1  Atabekova, N.V.1  Lipkin, V.M.1  Shmukler, B.E.1  Feshchenko, E.A.1  Rakitina, T.V.1  Khramtsov, N.V.1  Suslova, V.A.1  | |
[1] Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Muklaya 16110, 117871 GSP Moscow V-437, Russian Federation | |
关键词: Cyclic GMP phosphodiesterase; cDNA cloning; Polymerase chain reaction; Alternative splicing; Human retina; CAAX; Cys-aliphatic-aliphatic-X; | |
DOI : 10.1016/0014-5793(93)81003-I | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
cDNA clones encoding the β-subunit of the photoreceptor cGMP phosphodiesterase (PDE) were isolated from a human retina library and their sequence was determined. The encoded polypeptide consists of 854 amino acid residues with a calculated molecular mass of 98,416 Da. Alignment of the deduced amino acid sequence with the earlier analysed α-, β- and α'-subunits of bovine and mouse PDEs demonstrates a high homology. Two overlapping recombinant λ phage clones containing 26 kb of the human PDE β-subunit gene were isolated from the genomic library. A total nucleotide sequence of exons 4–22 of the PDE β-subunit gene was established which completely corresponded to the cDNA structure. According to sequence analysis no potential possibility for alternative splicing of the β-subunit gene was observed between exons 20 and 21 which led to the formation of the β'-subunit as described for mouse PDE. Polymerase chain reaction (PCR) experiments also confirm the absence of the PDE β'-subunit in human retina.
【 授权许可】
Unknown
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