Human Gi protein α‐subunit: deduction of amino acid structure from a cloned cDNA

FEBS Letters

Ho, Ye-Shih¹  Didsbury, John R.¹  Snyderman, Ralph¹ 

[1] Howard Hughes Medical Institute and Division of Rheumatology and Immunology, Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA

关键词: Adenylate cyclase-inhibiting G-protein;    cDNA cloning;    Nucleotide sequence;    Differentiation;   

DOI  :  10.1016/0014-5793(87)81428-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The amino acid sequence of the α-subunit of G_i, the human adenylate cyclase inhibiting GTP-binding protein, has been deduced from the nucleotide sequence of a DNA clone complementary to G_iα, mRNA from differentiated U937 cells. The cDNA encodes a polypeptide of 355 amino acids (M _r 40456). The amino acid sequence homology between human G_iα and rat, murine, and bovine G_iα is 98.6, 97.7 and 87.9% respectively. Differentiation of the U937 cells from monoblasts to monocyte-like cells resulted in a 3-fold increase in G_iα mRNA as well as a 3.6-fold increase in the 41 kDa pertussis toxin substrate presumed to be G_iα. Thus, increased levels of this G-protein are associated with monocyte differentiation and appear to be regulated transcriptionally.

【 授权许可】

Unknown