Cloning sequencing of Lol pI, the major allergenic protein of rye‐grass pollen

FEBS Letters

Singh, Mohan B.¹  Smith, Penelope M.³  Theerakulpisut, Piyada¹  Griffith, Irwin J.³  Davies, Sean¹  Knox, R.Bruce¹  Simpson, Richard J.²  Ward, Larry D.²  Avjioglu, Asil¹  Hough, Terryn¹  Pollock, Joanne³ 

[1] School of Botany, University of Melbourne, Parkville, Victoria 3052, Australia;Joint Protein Structure Laboratory, Ludwig Institute for Cancer Research (Melbourne Branch) and the Walter and Eliza Hall Institute for Medical Research, Parkville, Victoria 3052, Australia;ImmuLogic Pharmaceutical Corporation, One Kendall Square, Building 600, Cambridge, MA 02139, USA

关键词: Lolium perenne;    Allergen;    cDNA cloning;    Nucleotide sequence;    Amino acid sequence;   

DOI  :  10.1016/0014-5793(91)80151-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have isolated a full length cDNA clone encoding the major glycoprotein allergen Lol pI. The clone was selected using a combination of immunological screening of a cDNA expression library and PCR amplification of Lol pI-specific transcripts. Lol pI expressed in bacteria as a fusion protein shows recognition by specific IgE antibodies present in sera of grass pollen-allergic subjects. Northern analysis has shown that the Lol pI transcripts are expressed only in pollen of rye-grass. Molecular cloning of Lol pI provides a molecular genetic approach to study the structure—function relationship of allergens.

【 授权许可】

Unknown