Molecular cloning, expression and immunological characterisation of Lol p 5C, a novel allergen isoform of rye grass pollen demonstrating high IgE reactivity

FEBS Letters

Schäppi, George³  de Leon, Maria³  Mawdsley, David²  Gruehn, Sabine³  Rolland, Jennifer M.¹  O'Hehir, Robyn E.³  Suphioglu, Cenk³ 

[1] Department of Pathology and Immunology, Monash University Medical School, Alfred Hospital, Prahran, Vic. 3181, Australia;Ludwig Institute for Cancer Research, Royal Melbourne Hospital, Parkville, Vic. 3050, Australia;Department of Allergy, Asthma and Clinical Immunology, Monash University Medical School, Alfred Hospital, Commercial Road, Prahran, Vic. 3181, Australia

关键词: Lol p 5C;    Pollen allergen;    cDNA cloning;    Isoform;    Human IgE;    Lolium perenne;    aa;    amino acid(s);    ANGIS;    Australian National Genomic Information Service;    CRPE;    crude rye grass pollen extract;    IEF;    isoelectric focussing;    IPG;    immobilised pH gradient;    IUIS;    International Union of Immunological Societies;    mAb;    monoclonal murine antibody;    M r;    molecular mass;    NC;    nitrocellulose;    nLol p 5;    natural Lol p 5;    nt;    nucleotide(s);    ORF;    open reading frame;    pI;    isoelectric point;    RAST;    radioallergosorbent test;    rLol p 5;    recombinant Lol p 5;    TBP;    tributyl phosphine;    2D;    two-dimensional;   

DOI  :  10.1016/S0014-5793(99)01576-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel isoform of a major rye grass pollen allergen Lol p 5 was isolated from a cDNA expression library. The new isoform, Lol p 5C, shares 95% amino acid sequence identity with Lol p 5A. Both isoforms demonstrated shared antigenic activity but different allergenic activities. Recombinant Lol p 5C demonstrated 100% IgE reactivity in 22 rye grass pollen sensitive patients. In comparison, recombinant Lol p 5A showed IgE reactivity in less than 64% of the patients. Therefore, Lol p 5C represents a novel and highly IgE-reactive isoform allergen of rye grass pollen.

【 授权许可】

Unknown