FEBS Letters | |
Engineering of hypoallergenic mutants of the Brassica pollen allergen, Bra r 1, for immunotherapy | |
Okada, Takashi2  Bhalla, Prem L1  Swoboda, Ines1  Singh, Mohan B1  Toriyama, Kinya2  | |
[1] Plant Molecular Biology and Biotechnology Laboratory, Institute of Land and Food Resources, University of Melbourne, Parkville, Vic. 3052, Australia;Laboratory of Plant Breeding and Genetics, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan | |
关键词: Brassica rapa; Ca2+ binding; IgE reactivity; Mutagenesis analysis; Pollen allergen; Recombinant protein; | |
DOI : 10.1016/S0014-5793(98)00992-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The Brassica pollen allergen Bra r 1 belongs to a new family of Ca2+-binding proteins, characterized by the presence of two potential EF-hand calcium-binding domains. Disruption of these EF-hand motifs by amino acid substitutions demonstrated that both domains of Bra r 1 constitute functional Ca2+-binding sites. Calcium-binding deficient mutants displayed significantly reduced IgE-binding activity. Injection of these mutated Bra r 1 variants into a murine model system showed that mouse IgG raised against the mutants recognized native Bra r 1 in Brassica pollen extracts suggesting the potential use of the engineered allergens for effective immunotherapy.
【 授权许可】
Unknown
【 预 览 】
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RO201912020306465ZK.pdf | 245KB | download |