Engineering of hypoallergenic mutants of the Brassica pollen allergen, Bra r 1, for immunotherapy

FEBS Letters

Okada, Takashi²  Bhalla, Prem L¹  Swoboda, Ines¹  Singh, Mohan B¹  Toriyama, Kinya² 

[1] Plant Molecular Biology and Biotechnology Laboratory, Institute of Land and Food Resources, University of Melbourne, Parkville, Vic. 3052, Australia;Laboratory of Plant Breeding and Genetics, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan

关键词: Brassica rapa;    Ca2+ binding;    IgE reactivity;    Mutagenesis analysis;    Pollen allergen;    Recombinant protein;   

DOI  :  10.1016/S0014-5793(98)00992-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Brassica pollen allergen Bra r 1 belongs to a new family of Ca²⁺-binding proteins, characterized by the presence of two potential EF-hand calcium-binding domains. Disruption of these EF-hand motifs by amino acid substitutions demonstrated that both domains of Bra r 1 constitute functional Ca²⁺-binding sites. Calcium-binding deficient mutants displayed significantly reduced IgE-binding activity. Injection of these mutated Bra r 1 variants into a murine model system showed that mouse IgG raised against the mutants recognized native Bra r 1 in Brassica pollen extracts suggesting the potential use of the engineered allergens for effective immunotherapy.

【 授权许可】

Unknown