Inhibition of myeloid differentiation by inhibitors of ADP‐ribosylation

FEBS Letters

Wu, Fu-Mei¹  Zaun, M.Rebecca¹  Wu, Ming-chi¹ 

[1] Department of Biochemistry, Texas College of Osteopathic Medicine, University of North Texas, Denton, TX 76203, USA

关键词: Leukemia;    Cell differentiation;    Colony-stimulating factor;    ADP-ribosylation;    BADGH;    benzylaminododecylguanidine hydrochloride;    MBAMG;    p-methoxylbenzylaminodecamethylene guanidine sulfate;    GM-CSF;    granulocyte-macrophage colony-stimulating factor;    G-CSF;    granulocyte colony-stimulating factor;    DME-S;    Dulbecco's modified Eagle's with serum;    CSF;    colony-stimulating factor;    E-MLCM;    endotoxin-treated mouse lung conditioned medium;   

DOI  :  10.1016/0014-5793(89)80558-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Inhibitors of ADP-ribosylation inhibited the myeloid differentiation of murine myelomonocytic leukemia WEHI-3BD⁺ cells induced by granulocyte colony-stimulating factor. Benzamide, at 2.0 mM, inhibited 50% of the WEHI-3BD⁺ cell differentiation but had no significant effect on the proliferation. However, benzylaminododecylguanine hydrochloride and p-methoxylbenzylaminodecamethylene guanidine sulfate at 2.0 and 2.2 μM, respectively, inhibited 50% of proliferation but had no effect at all on differentiation. The differential effects of inhibitors provide a model to study the role of ADP-ribosylation in myeloid differentiation.

【 授权许可】

Unknown