Preparation and neutralization characteristics of an antibody to the factor inducing differentiation of mouse myeloid leukemic cells

FEBS Letters

Yamamoto-Yamaguchi, Y.¹  Tomida, M.¹  Hozumi, M.¹ 

[1] Department of Chemotherapy, Saitama Cancer Center Research Institute, Ina-machi, Kitaadachi-gun, Saitama-ken 362, Japan

关键词: Myeloid leukemic cell;    Differentiation factor;    Antibody;    Bone marrow cell;    Colony-stimulating factor;    D-factor;    differentiation-stimulating factor;    MGI;    macrophage and granulocyte inducer;    CSF;    colony-stimulating factor;    G-CSF;    granulocyte colony-stimulating factor;    CM;    conditioned medium;   

DOI  :  10.1016/0014-5793(83)80087-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mouse myeloid leukemic M1 cells can be induced to differentiate into macrophages and granulocytes in vitro by a protein inducer (D-factor) in body fluids and conditioned media from various cells and tissues. Antiserum to D-factor was prepared by immunizing a rabbit with D-factor partially purified from conditioned medium of mouse L929 cells. At 1:20 dilution, the antiserum nearly completely suppressed the activities of various D-factor preparations from mice and partially suppressed the activities of preparations from rats and hamsters. It did not cross-react with factors stimulating colony formation of macrophages and granulocytes from normal bone marrow cells.

【 授权许可】

Unknown