FEBS Letters | |
Preparation and neutralization characteristics of an antibody to the factor inducing differentiation of mouse myeloid leukemic cells | |
Yamamoto-Yamaguchi, Y.1  Tomida, M.1  Hozumi, M.1  | |
[1] Department of Chemotherapy, Saitama Cancer Center Research Institute, Ina-machi, Kitaadachi-gun, Saitama-ken 362, Japan | |
关键词: Myeloid leukemic cell; Differentiation factor; Antibody; Bone marrow cell; Colony-stimulating factor; D-factor; differentiation-stimulating factor; MGI; macrophage and granulocyte inducer; CSF; colony-stimulating factor; G-CSF; granulocyte colony-stimulating factor; CM; conditioned medium; | |
DOI : 10.1016/0014-5793(83)80087-8 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Mouse myeloid leukemic M1 cells can be induced to differentiate into macrophages and granulocytes in vitro by a protein inducer (D-factor) in body fluids and conditioned media from various cells and tissues. Antiserum to D-factor was prepared by immunizing a rabbit with D-factor partially purified from conditioned medium of mouse L929 cells. At 1:20 dilution, the antiserum nearly completely suppressed the activities of various D-factor preparations from mice and partially suppressed the activities of preparations from rats and hamsters. It did not cross-react with factors stimulating colony formation of macrophages and granulocytes from normal bone marrow cells.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020283915ZK.pdf | 399KB | download |