Plasminogen activator activity in differentiating leukemia cells

FEBS Letters

Nachshon, I.¹  Fibach, E.²  Mayer, M.¹  Kidron, M.¹ 

[1] Departments of Biochemistry, Hebrew University-Hadassah Medical School, PO Box 24035, Jerusalem 91240, Israel;Hematology, Hadassah University Hospital, Mount Scopus, PO Box 24035, Jerusalem 91240, Israel

关键词: Plasminogen activator;    Myeloid leukemic cell;    Differentiation;    Macrophage;    PA;    plasminogen activator;    TPA;    12-O-tetradecanoyl-phorbol-13-acetate;   

DOI  :  10.1016/0014-5793(84)80982-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Plasminogen activator (PA) activity of human promyelocytic leukemia cell line HL-60 was assayed by following the conversion of plasminogen to plasmin and the plasmin-mediated hydrolysis of ¹⁴C-labeled globin. When HL-60 cells were induced to differentiate into macrophages by 12-O-tetradecanoyl-phorbol-13-acetate (TPA), cell-associated PA activity and secretion of PA into the conditioned medium increased profoundly. PA activity increased earlier and as a result of lower concentrations of TPA than the ability of the cells to adhere. Exposure to 10⁻⁶ M dexamethasone did not prevent TPA-induced adherence and produced a slight inhibition of cellular PA activity. These findings imply that TPA-induced differentiation of HL-60 cells to macrophage-like cells is associated with induction of PA activity.

【 授权许可】

Unknown