FEBS Letters | |
Characterization of a factor inducing differentiation of mouse myeloid leukemic cells purified from conditioned medium of mouse Ehrlich ascites tumor cells | |
Tomida, Mikio1  Yamamoto-Yamaguchi, Yuri1  Hozumi, Motoo1  | |
[1] Department of Chemotherapy, Saitama Cancer Center Research Institute, Ina-machi, Kitaadachi-gun, Saitama-Ken 362, Japan | |
关键词: Myeloid leukemic cell; Macrophage; Differentiation factor; Structure; Glycoprotein; Antigenicity; abr]D-factor; differentiation-inducing factor; MGI; macrophage and granulocyte inducer; M-; G- and GM-CSF; macrophage; granulocyte and granulocyte-macrophage colony-stimulating factors; respectively; HPLC; high-performance liquid chromatography; CM; conditioned medium; | |
DOI : 10.1016/0014-5793(84)80619-5 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
A factor inducing differentiation of mouse myeloid leukemic cells (M1) into macrophages was purified to apparent homogeneity from 168 1 of CM of Ehrlich ascites tumor cells. The purified factor was halt-maximally active at 2 × 10−11 M. The factor was analyzed by radioiodination, SDS-polyacrylamide gel electrophoresis and autoradiography. Its M r was 40000–50000. On reduction, the factor lost activity, but showed no subunit structure. Treatment of the factor with endo-β-N-acetylglucosaminidase F, but not endo-β-N-acetylglucosaminidase H, gave rise to a molecule of M r 20000–28000. The activity of the factor from Ehrlich cells was completely neutralized by antiserum to the factor of M r 50000–70000 from mouse fibroblast L929 cells.
【 授权许可】
Unknown
【 预 览 】
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