Characterization of a factor inducing differentiation of mouse myeloid leukemic cells purified from conditioned medium of mouse Ehrlich ascites tumor cells

FEBS Letters

Tomida, Mikio¹  Yamamoto-Yamaguchi, Yuri¹  Hozumi, Motoo¹ 

[1] Department of Chemotherapy, Saitama Cancer Center Research Institute, Ina-machi, Kitaadachi-gun, Saitama-Ken 362, Japan

关键词: Myeloid leukemic cell;    Macrophage;    Differentiation factor;    Structure;    Glycoprotein;    Antigenicity;    abr]D-factor;    differentiation-inducing factor;    MGI;    macrophage and granulocyte inducer;    M-;    G- and GM-CSF;    macrophage;    granulocyte and granulocyte-macrophage colony-stimulating factors;    respectively;    HPLC;    high-performance liquid chromatography;    CM;    conditioned medium;   

DOI  :  10.1016/0014-5793(84)80619-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A factor inducing differentiation of mouse myeloid leukemic cells (M1) into macrophages was purified to apparent homogeneity from 168 1 of CM of Ehrlich ascites tumor cells. The purified factor was halt-maximally active at 2 × 10⁻¹¹ M. The factor was analyzed by radioiodination, SDS-polyacrylamide gel electrophoresis and autoradiography. Its M _r was 40000–50000. On reduction, the factor lost activity, but showed no subunit structure. Treatment of the factor with endo-β-N-acetylglucosaminidase F, but not endo-β-N-acetylglucosaminidase H, gave rise to a molecule of M _r 20000–28000. The activity of the factor from Ehrlich cells was completely neutralized by antiserum to the factor of M _r 50000–70000 from mouse fibroblast L929 cells.

【 授权许可】

Unknown