Adrenomedullin production is correlated with differentiation in human leukemia cell lines and peripheral blood monocytes

FEBS Letters

Minamino, Naoto¹  Kubo, Atsushi¹  Kangawa, Kenji¹  Dohi, Kazuhiro²  Isumi, Yoshitaka¹  Matsuo, Hisayuki¹ 

[1]National Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka 565-8565, Japan

[2]First Department of Internal Medicine, Nara Medical University, Shijo, Kashihara, Nara 634-0813, Japan

关键词: Adrenomedullin;    Monocyte;    Macrophage;    Granulocyte;    Differentiation;    AM;    adrenomedullin;    EC;    endothelial cell;    VSMC;    vascular smooth muscle cell;    IL-1;    interleukin-1;    TNF-α;    tumor necrosis factor-α;    LPS;    lipopolysaccharide;    RA;    retinoic acid;    IFN-γ;    interferon-γ;    DMEM;    Dulbecco's modified Eagle's medium;    BSA;    bovine serum albumin;    FCS;    fetal calf serum;    RIA;    radioimmunoassay;    IR;    immunoreactive;    PBS;    phosphate-buffered saline;    FITC;    fluorescein isothiocyanate;    DiI-acLDL;    1;    1′-dioctadecyl-3;    3;    3′;    3′-tetramethylindo-carbocyanine perchorate acetylated low density lipoprotein;   

DOI  :  10.1016/S0014-5793(98)00349-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We demonstrated that adrenomedullin (AM) is produced and secreted from human leukemia cell lines (THP-1 and HL-60) as well as peripheral blood granulocytes, lymphocytes, monocytes and monocyte-derived macrophages. Immunoreactive AM accumulated in the culture media of THP-1 and HL-60 cells increased according to their differentiation into macrophage-like cells. Retinoic acid exerted synergistic effects on AM secretion from THP-1 and HL-60 cells when administered with tumor necrosis factor-α, lipopolysaccharide or 12-O-tetradecanoyl phorbol-13-acetate. AM was shown to increase the scavenger receptor activity on THP-1 cells. Thus, monocytes/macrophages should be recognized as sources of AM, and the secreted AM may modulate the function of macrophages.

【 授权许可】

Unknown