Detection of BCR-ABL Fusion mRNA Using Reverse Transcriptase Loop-mediated Isothermal Amplification

Dugan, L. C. ; Hall, S. ; Kohlgruber, A. ; Urbin, S. ; Torres, C. ; Wilson, P.

Lawrence Livermore National Laboratory

关键词: Amplification;    Patients;    59 Basic Biological Sciences;    Leukemia;    Myeloid Leukemia;   

DOI  :  10.2172/1034502
RP-ID  :  LLNL-TR-520096
RP-ID  :  W-7405-ENG-48
RP-ID  :  1034502

美国|英语

来源: UNT Digital Library

【 摘 要 】

RT-PCR is commonly used for the detection of Bcr-Abl fusion transcripts in patients diagnosed with chronic myelogenous leukemia, CML. Two fusion transcripts predominate in CML, Br-Abl e13a2 and e14a2. They have developed reverse transcriptase isothermal loop-mediated amplification (RT-LAMP) assays to detect these two fusion transcripts along with the normal Bcr transcript.