Field Deployable DNA analyzer

Wheeler, E ; Christian, A ; Marion, J ; Sorensen, K ; Arroyo, E ; Vrankovich, G ; Hara, C ; Nguyen, C

Lawrence Livermore National Laboratory

关键词: Amplification;    Packed Beds;    Sample Preparation;    Dna;    59 Basic Biological Sciences;   

DOI  :  10.2172/917501
RP-ID  :  UCRL-TR-209651
RP-ID  :  W-7405-ENG-48
RP-ID  :  917501

美国|英语

来源: UNT Digital Library

【 摘 要 】

This report details the feasibility of a field deployable DNA analyzer. Steps for swabbing cells from surfaces and extracting DNA in an automatable way are presented. Since enzymatic amplification reactions are highly sensitive to environmental contamination, sample preparation is a crucial step to make an autonomous deployable instrument. We perform sample clean up and concentration in a flow through packed bed. For small initial samples, whole genome amplification is performed in the packed bed resulting in enough product for subsequent PCR amplification. In addition to DNA, which can be used to identify a subject, protein is also left behind, the analysis of which can be used to determine exposure to certain substances, such as radionuclides. Our preparative step for DNA analysis left behind the protein complement as a waste stream; we determined to learn if the proteins themselves could be analyzed in a fieldable device. We successfully developed a two-step lateral flow assay for protein analysis and demonstrate a proof of principle assay.