Rapid Automated Sample Preparation for Biological Assays

Shusteff, M

Lawrence Livermore National Laboratory

关键词: Sample Preparation;    Dna;    Separation Processes;    59 Basic Biological Sciences;    Extraction;   

DOI  :  10.2172/1021552
RP-ID  :  LLNL-TR-472231
RP-ID  :  W-7405-ENG-48
RP-ID  :  1021552

美国|英语

来源: UNT Digital Library

【 摘 要 】

Our technology utilizes acoustic, thermal, and electric fields to separate out contaminants such as debris or pollen from environmental samples, lyse open cells, and extract the DNA from the lysate. The objective of the project is to optimize the system described for a forensic sample, and demonstrate its performance for integration with downstream assay platforms (e.g. MIT-LL's ANDE). We intend to increase the quantity of DNA recovered from the sample beyond the current {approx}80% achieved using solid phase extraction methods. Task 1: Develop and test an acoustic filter for cell extraction. Task 2: Develop and test lysis chip. Task 3: Develop and test DNA extraction chip. All chips have been fabricated based on the designs laid out in last month's report.