The synthesis of human α‐2‐HS glycoprotein is down‐regulated by cytokines in hepatoma HepG2 cells

FEBS Letters

Lebreton, Jean-Pierre¹  Julen, Nathalie¹  Daveau, Maryvonne¹  Amaud, Philippe²  Hiron, Martine¹ 

[1] INSERM Unité 78, BP 73, 76233 Bois-Guillaume Cedex, France;Laboratory of Molecular Genetics, Medical University of South Carolina, Charleston, SC, USA

关键词: Acute-phase protein;    Glycoprotein α-2-HS;    Albumin;    Interleukin-6;    Interleukin-1;    BSF-2;    B-cell differentiation factor;    IFN-β2;    interferon-β2;    FCS;    fetal calf scrum;    CM;    conditioned medium;    ITG;    guanidine isothiocyanate;   

DOI  :  10.1016/0014-5793(88)81059-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The regulation of the synthesis of α-2-HS glycoprotein (AHSG) by inflammatory mediators from activated monocytes was studied on the human hepatoma cell line HepG2 and compared to that of albumin. Monocyte-conditioned medium, recombinant human interleukin-6 (rhIL6) and interleukin-1β(rhIL1β) all down-regulated the synthesis of AHSG. This decrease was found both at the protein and the mRNA level. The most efficient mediator was the monocyte-conditioned medium, when rhIL1β was found to be less efficient than rhIL6. The combination of rhIL6 and rhIL1β resulted in an additive down-regulation of the AHSG mRNA levels. Similar results were obtained with albumin. These data indicate that AHSG is a negative acute-phase protein whose synthesis is regulated by cytokines in a manner similar to that of albumin.

【 授权许可】

Unknown