Recombinant human interleukin‐6 (IL‐6/BSF‐2/HSF) regulates the synthesis of acute phase proteins in human hepatocytes

FEBS Letters

Castell, José V.³  David, Martina³  Gómez-Lechón, Maria J.²  Kishimoto, Tadamitsu¹  Heinrich, Peter C.³  Hirano, Toshio¹ 

[1] Institute for Molecular and Cellular Biology, Division of Immunology, Osaka University, 1-3, Yamada-oka, Suita, Osaka 565, Japan;Centro de Investigación, Hospital La Fe, INSALUD, Avda de Campanar, 21, E-46009 Valencia, Spain;Biochemisches Institut, Universität Freiburg, Hermann-Herder-Str. 7, D-7800 Freiburg, FRG

关键词: Acute-phase protein;    Interleukin-6;    Hepatocyte stimulating factor;    B-cell stimulatory factor 2;    (Human hepatocyte);    BSF-2;    B-cell stimulatory factor 2;    HSF;    hepatocyte-stimulating factor;    IL-6;    interleukin-6;    rhIL-6;    recombinant human interleukin-6;   

DOI  :  10.1016/0014-5793(88)80766-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Recombinant human IL-6 (rhIL-6) is a potent inducer of the synthesis of acute phase proteins in adult human hepatocytes. A wide spectrum of acute phase proteins is regulated by this mediator. After labeling of rhIL-6 stimulated human hepatocytes with [³⁵S]methionine acute phase protein synthesis was measured by immunoprecipitation. Serum amyloid A, C-reactive protein, haptoglobin, α₁-antichymotrypsin and fibrinogen were strongly induced (26-, 23-, 8.6-, 4.6- and 3.8-fold increases, respectively). Moderate increases were found for α₁-antitrypsin (2.7-fold) and α₁-acid glycoprotein (2.7-fold). RhIL-6 had no effect on α₁-macroglobulin, whereas fibronectin, albumin and transferrin decreased to 64, 56 and 55% of controls. In the cases of serum amyloid A, haptoglobin, α₁-antichymotrypsin, α₁-antitrypsin and α₁-acid glycoprotein, dexamethasone enhanced the action of rhIL-6. We conclude that rhIL-6 controls the acute phase response in human liver cells.

【 授权许可】

Unknown