Recombinant human interleukin 6 (B‐cell stimulatory factor 2) is a potent inducer of differentiation of mouse myeloid leukemia cells (M1)

FEBS Letters

Taniyama, Tadayoshi²  Onozaki, Kikuo⁴  Suda, Tatsuo³  Kishimoto, Tadamitsu¹  Akiyama, Yukio⁵  Miyaura, Chisato³  Hirano, Toshio¹ 

[1] Division of Immunology, Institute for Molecular and Cellular Biology, Osaka University, Osaka 565, Japan;Department of Cellular Immunology, National Institute of Health, Shinagawa, Tokyo 141 Japan;Department of Biochemistry, School of Dentistry, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142, Japan;Department of Microbiology, Institute of Basic Medical Sciences, The University of Tsukuba, Tsukuba, Ibaraki 305, Japan;Central Research Laboratories, Ajinomoto Co. Ltd, Kawasaki-ku, Kawasaki 210 Japan

关键词: B-cell stimulatory factor 2;    Interleukin-6;    Vitamin D3;    Growth inhibition;    Differentiation;    (Mouse myeloid leukemia cell);   

DOI  :  10.1016/0014-5793(88)81293-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Recombinant human interleukin 6 (IL-6), a lymphokine involved in the final differentiation of activated B-cells into antibody-forming cells, greatly suppressed proliferation and induced differentiation of murine myeloid leukemia cells (M1) into mature macrophage-like cells. When M1 cells were treated with IL-6, their growth was completely arrested as early as on day 2, and they were induced to differentiate morphologically into macrophage-like cells. Differentiation-associated properties such as phagocytic activity, adherence to the dish surface, Fc and C3 receptors, were also induced within 24 h by IL-6, and they reached their respective maximal levels on day 2 or 3. The potency of IL-6 in suppressing proliferation and inducing differentiation was much greater than that of 1α,25-dihydroxyvitamin D₃ one of the most potent inducers of M1 cells. The present report indicates that IL-6 is involved in the differentiation of not only B-cells but also myeloid leukemia cells.

【 授权许可】

Unknown