Ciliary neurotrophic factor induces acute‐phase protein expression in hepatocytes

FEBS Letters

Schooltink, Heidi¹  Stoyan, Tanja¹  Heinrich, Peter C.¹  Roeb, Elke¹  Rose-John, Stefan¹ 

[1] Institut für Biochemie, der RWTH Aachen, Pauwelsstr. 30, D-5100 Aachen, Germany

关键词: Acute-phase protein;    Ciliary neurotrophic factor;    Hepatocyte;    Leukemia inhibitory factor;    Interleukin-6;    ACT;    α1-antichymotrypsin;    α2M;    α2-macroglobulin;    CNTF;    ciliary neurotrophic factor;    FIB;    β-fibrinogen;    HPT;    haptoglobin;    IL;    interleukin;    LIF;    leukemia inhibitory factor;    OSM;    oncostatin M;    rh;    recombinant human;   

DOI  :  10.1016/0014-5793(92)81489-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

During inflammatory states, hepatocytes are induced to synthesize and secrete a group proteins called acute-phase proteins. It has recently been shown that besides interleukin-6 (IL-6) related cytokines such as leukemia inhibitory factor, oncostation M and interleukin-11 are also mediators of the degatic acute-phase response. All these mediators belong to the dematoqaietic family of α-helical cytokines. Here we show that an additional member of this cytokine family, ciliary neurotrophic factor (CNTF), induces the hepatic acute-phase protein genes heptaglobin α₁-antichymotrypsin, α₂-macroglobulin and β-fibrinogen in human hepatoma cells (HepG2) and in primary rat hepatocytes with a time course and dose-response comparable with that of IL-6. Our next aim was to define the receptor components used by CNTF on hepatic cells. Using a cell-free binding assay we exclude that CNTF binds to the 80 kDa IL-6 receptor, a protein with significant homology to the CNTF receptor which has recently been cloned from neuroblastoma cells. In human hepatoma cells (Hep3B) which lack the leukemia inhibitory factor receptor. CNTF was not able to induce acute-phase protein synthesis, indicating that this receptor protein may be part of the functional CNTF receptor on hepatic cells.

【 授权许可】

Unknown