Induction of rat α2‐macroglobulin in vivo and in hepatocyte primary cultures: synergistic action of glucocorticoids and a Kupffer cell‐derived factor

FEBS Letters

Ueberberg, Heinz²  Northemann, Wolgang³  Bauer, Joachim³  Heinrich, Peter C.³  Northoff, Gerd-Hinrich⁴  Birmelin, Manfred³  Tran-Thi, Thuy-Anh¹  Decker, Karl³ 

[1] Dr. Karl Thomae GmbH. Birkendorf er-Str. 65, D-7950 Biberach, FRG;Biochemisches Institut, Universität Freiburg, Hermann-Herder-Str. 7, D-7800 FreiburgGermany;Blutspendezentrale des DRK, Oberer Eselsberg, D-7900 UlmGermany

关键词: Acute-phase protein;    α2-Macroglobulin;    Glucocorticoid;    Hepatocyte-stimulating factor;    Kupffer cell;    α2M;    α2-macroglobulin;    α1AGP;    α1-acid glycoprotein;    AT III;    antithrombin III;    α1 APG;    α1-acute-phase globulin;    α1PI;    α1-proteinase inhibitor;    TF;    transferrin;    LPS;    lipopolysaccharide;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(84)80987-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Turpentine injection into rats elicits enhanced secretion of acute phase proteins inducing α₂-macroglobulin (α₂M). Hypophysectomized rats, however, do not respond in this way unless dexamethasone is given together with turpentine. On the other hand, dexamethasone injection alone did not result in an induction of α₂M synthesis. When a medium of Kupffer cell cultures was added to hepatocytes, a dose-dependent stimulation of α₂M synthesis of up to 4-fold after 10–12 h was observed. However, the presence of low concentrations (10⁻⁹ M) of dexamethasone was essential for the stimulatory effect. We conclude that the acute phase induction of α₂M in hepatocytes requires the synergistic action of glucocorticoids and a non-dialysable factor secreted by Kupffer cells.

【 授权许可】

Unknown