Human heart cytochrome c oxidase subunit VIII Purification and determination of the complete amino acid sequence

FEBS Letters

Demol, Hans¹  Dekker, Henk L.²  Muijsers, Anton O.²  Van Beeumen, Jozef J.¹  Van Kuilenburg, André B.P.² 

[1] Laboratory of Microbiology and Microbial Genetics, University of Ghent, Ledeganckstraat 35, B-9000 Ghent, Belgium;Laboratory of Biochemistry, University of Amsterdam, PO Box 20151, 1000 HD Amsterdam, The Netherlands

关键词: Cytochrome oxidase;    Subunit VIII;    Amino acid sequence;    (Human);    TEA;    triethylamine;    TFA;    trifluoroacetic acid;    HPLC;    high-performance liquid chromatography;   

DOI  :  10.1016/0014-5793(88)80353-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Subunit VIII was purified from a preparation of the human heart cytochrome c oxidase and its complete amino acid sequence was determined. The sequence proved to be much more related to that of the bovine liver oxidase subunit VIII than to that found in bovine heart. Our finding of a ‘liver-type’ subunit VIII in the human heart enzyme suggests that either there are no isoforms of human subunit VIII or the human oxidase does not show the type of tissue specificity that has been reported for the oxidase in other mammals.

【 授权许可】

Unknown