Identification of a Glu > Lys substitution in the activation segment of human pepsinogen A‐3 and ‐5 isozymogens by peptide mapping using endoproteinase Lys‐C

FEBS Letters

Bank, Ruud A.¹  Arwert, Fre¹  Pronk, Jan C.¹  Meuwissen, Stephan G.M.²  Crusius, Bart C.¹  Zwiers, Toon¹ 

[1] Institute of Human Genetics, Free University, Amsterdam, The Netherlands;Department of Gastroenterology, Free University, Amsterdam, The Netherlands

关键词: Pepsinogen;    Peptide mapping;    Amino acid sequence;    Evolution;    (Human);   

DOI  :  10.1016/0014-5793(88)80235-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The isozymogens PGA-3 and PGA-5 of human pepsinogen A were digested with endoproteinase Lys-C. The peptides were separated by reverse-phase HPLC. PGA-5 showed a peak strongly absorbing at 254 nm absent in PGA-3. Analysis of amino acid composition using the Pico-Tag methodology combined with DABITC-sequencing reveals the sequence Tyr-Phe-Pro-Gln-Trp-Lys (peptide 37–43 of the activation segment). This confirms a study at the DNA level by our group [16] suggesting a Glu > Lys mutation at position 43 in the activation segment of PGA-5. Furthermore, it is proposed that the number of genetic variants of PGA is higher than is actually seen by electrophoresis.

【 授权许可】

Unknown