Amino acid sequences of the human kidney cathepsins H and L

FEBS Letters

Machleidt, Werner²  Popović, Tatjana¹  Ritonja, Anka¹  Kotnik, Matjaž¹  Turk, Vito¹ 

[1] Department of Biochemistry, J. Stefan Institute, Jamova 39, 61000 Ljubljana, Yugoslavia;Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie der Universität München, Goethestrasse 33, D-8000 München 2, FRG

关键词: Cathepsin H;    Cathepsin L;    Amino acid sequence;    Procathepsin;    Cysteine proteinase;    (Human);   

DOI  :  10.1016/0014-5793(88)80028-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The complete amino acid sequences of human kidney cathepsin H (EC 3.4.22.16) and human kidney cathepsin L (EC 3.4.22.15) were determined. Cathepsin H contains 230 residues and has an M _r of 25 116. The sequence was obtained by sequencing the light, heavy and mini chain and the peptides produced by cyanogen bromide cleavage of the single-chain form of the enzyme. The glycosylated mini chain is a proteolytic fragment of the propeptide of cathepsin H. Human cathepsin L has 217 amino acid residues and an M _r of 23720. Its amino acid sequence was deduced from N-terminal sequences of the heavy and light chains and from the sequences of cyanogen bromide fragments of the heavy chain. The fragments were aligned by comparison with known sequences of cathepsins H and L from other species. Cathepsins H and L exhibit a high degree of sequence homology to cathepsin B (EC 3.4.22.1) and other cysteine proteinases of the papain superfamily.

【 授权许可】

Unknown