Biosyntheses and processing of lysosomal cysteine proteinases in rat macrophages

FEBS Letters

Katunuma, Nobuhiko²  Tsukahara, Toshifumi¹  Hara, Kenji²  Kominami, Eiki² 

[1] National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo 187, Japan;Division of Enzyme Chemistry, Institute for Enzyme Research, The University of Tokushima, Tokushima 770, Japan

关键词: Cathepsin B;    Cathepsin H;    Cathepsin L;    Intracellular processing;    (Rat macrophage);    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(88)80736-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The intracellular processing and release of three lysosomal cysteine proteinases, cathepsin B, H and L, by rat peritoneal macrophages were investigated by pulse-chase experiments. Newly synthesized procathepsins B (39 kDa), H(41 kDa) and L (39 kDa) after 15 min labeling were processed to the mature, single-chain enzymes within 1 h. The single-chain forms of cathepsin B, H and L were further processed to two-chain forms at different rates: conversion of cathepsin L to the two-chain form was rapid, whereas the conversions cathepsin B and H took at least 6 h. Macrophages released 30% of the procathepsins B and L, and 10% of the procathepsin H.

【 授权许可】

Unknown