Influence of 2‐chloroadenosine on the nucleotide content of isolated rat hepatocytes

FEBS Letters

Claeyssens, Sophie²  Chedeville, Arlette²  Hamet, Michèle¹  Basuyau, Jean-Pierre²  Lavoinne, Alain² 

[1] INSERM U 75, Faculté de Médecine Necker-Enfants Malades, 156 rue de Vaugirard, 76016 Paris, France;Groupe de Biochimie et de Physiopathologie Digestive et Nutritionnelle, UER Médecine et Pharmacie de Rouen, Avenue de l'Université, BP 97, 76800 Saint Etienne du Rouvray France

关键词: 2-Chloroadenosine;    Adenosine;    Nucleotide triphosphate;    HPLC;    Hepatocyte;   

DOI  :  10.1016/0014-5793(88)80761-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

2-Chloroadenosine is presumably a non-metabolizable analogue of adenosine; however, this compound induced an increase in the enzymatically measured nucleotide content of isolated rat hepatocytes. HPLC separation and spectral analysis of the peaks showed that this increase may be related to the formation of 2-chloro nucleotides and that the 2-chloro nucleotides appeared in the first minutes of the incubation period. These results demonstrate that 2-chloroadenosine may be metabolized by phosphorylation in rat liver cells.

【 授权许可】

Unknown