Immunochemical characterization of the modulator protein of the ATP,Mg‐dependent protein phosphatase

FEBS Letters

Merlevede, Wilfried¹  Vandenheede, Jackie R.¹  Abeele, Carline Vanden¹ 

[1] Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit te Leuven, B-3000 Leuven, Belgium

关键词: ATP;    Mg-dependent protein phosphatase;    Modulator protein;    Polyclonal antibody;   

DOI  :  10.1016/0014-5793(88)80410-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Polyclonal antibodies raised against the modulator protein of the ATP,Mg-dependent protein phosphatase completely neutralize all known properties of the purified modulator: inhibition or inactivation of the phosphatase catalytic subunit as well as the kinase F_A-mediated activation of the ATP,Mg-dependent phosphatase. They do not cross-react with phosphoinhibitor-1 or the phosphatase catalytic subunit. Direct analysis of boiled or unboiled skeletal muscle extracts by Western blotting reveals a 32 kDa polypeptide corresponding to the modulator protein as the most dominant protein staining band.

【 授权许可】

Unknown