On the dephosphorylation of the ATP,Mg‐dependent protein phosphatase modulator

FEBS Letters

Merlevede, Wilfried¹  Vandenheede, Jackie R.¹  Abeele, Carline Vanden¹ 

[1] Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit te Leuven, B-3000 Leuven, Belgium

关键词: ATP;    Mg-dependent protein phosphatase;    Modulator protein;    Inhibitor-1;    Protein kinase FA;    Mg2+;   

DOI  :  10.1016/0014-5793(87)80708-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The dephosphorylation of the modulator subunit is an essential step in the kinase F_A-mediated activation of the ATP,Mg-dependent protein phosphatase. Mg²⁺ is implicated in this autocatalytic dephosphorylation which is not effected by the addition of phosphoinhibitor-1. Dephosphorylation of free modulator by the catalytic subunit is also largely Mg²⁺-dependent but can be abolished by phosphoinhibitor-1 in concentrations comparable to the amount of modulator used as substrate (micromolar). The phosphorylase phosphatase activity of the catalytic subunit is inhibited by nanomolar concentrations of phosphoinhibitor-1 and is completely independent of divalent cations.

【 授权许可】

Unknown