Isolation of human pancreastatin fragment containing the active sequence from a glucagonoma

FEBS Letters

Bretherton-Watt, D.²  Matsuo, H.¹  Bloom, S.R.²  Minamino, N.¹  Ghatei, M.A.²  Sekiya, K.² 

[1]Department of Biochemistry, Miyazaki Medical College, Kiyotake, Miyazaki 889-16, Japan

[2]Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, London W12 0HS, England

关键词: Pancreastatin;    Glucagonoma;    Sequence analysis;    (Human);    PS-LI;    pancreastatin-like immunoreactivity;    DDT;    Dl-dithiothreitol;    RIA;    radioimmunoassay;    BSA;    bovine serum albumin;    HPLC;    high-pressure liquid chromatography;    ACN;    acetonitrile;    TFA;    trifluoroacetic acid;    FPLC;    fast-protein liquid chromatography;   

DOI  :  10.1016/0014-5793(88)80606-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The primary structure of a human pancreastatin-like peptide was determined from a pancreatic glucagonoma. The 28-amino acid peptide was identified using a specific antibody raised against porcine pancreastatin 1–49 and showed a 75% sequence homology with porcine pancreastatin 22–49 and bovine chromogranin A 267–294. Several forms of pancreastatin-like immunoreactivity were found in human endocrine tumors of which the purified peptide was the smallest and contained the active sequence of pancreastatin.

【 授权许可】

Unknown