The molecular cloning of the chromogranin A‐like precursor of β‐granin and pancreastatin from the endocrine pancreas

FEBS Letters

Kastern, William¹  Hutton, John C.²  Nielsen, Egon¹ 

[1] Hagedorn Research Laboratory, Niels Steensensvej 6, Gentofte DK2850, Denmark;Department of Clinical Biochemistry, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QR, England

关键词: Chromogranin A;    β-Granin;    Pancreastatin;    cDNA;    OPA repeat;    (Pancreatic islet);   

DOI  :  10.1016/0014-5793(88)80036-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cDNA encoding the precursor form of the chromogranin A-related proteins, β-granin and pancreastatin, was obtained by immune screening of rat insulinoma and pancreatic islet cDNA libraries. The sequence was virtually identical to that of rat adrenal chromogranin A, suggesting that the different molecular forms of chromogranin A immunoreactivity found in adrenal medulla and endocrine pancreas are related to differences in post-translational proteolytic processing. The rat chromogranin A, unlike its bovine and human counterparts, contained a 20-residue glutamine sequence inserted within the N-terminal β-granin sequence. Although the encoding CA(G/A) repeat recurs frequently in the rat genome, the rat chromogranin A molecule appears to be the product of a single gene and mRNA transcript.

【 授权许可】

Unknown