The importance of the C‐terminal amide structure of rat pancreastatin to inhibit pancreatic exocrine secretion

FEBS Letters

Kitani, Kenichi¹  Fujii, Nobutaka¹  Funakoshi, Akihiro¹  Funakoshi, Susumu¹  Miyasaka, Kyoko¹  Tamamura, Hirokazu¹ 

[1]First Laboratory of Clinical Physiology, Tokyo Metropolitan Institute of Gerontology, Kyoto University, Kyoto 606 Japan

关键词: Pancreastatin;    C-terminal amide;    (Rat Pancreas);   

DOI  :  10.1016/0014-5793(90)81392-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A C-terminal fragment of rat pancreastatin, a 26 residue peptide amide and a fragment without a C-terminal amide were synthesized by Fmoc-based solid phase methods and their biological activities were compared. The rat C-terminal fragment inhibited pancreatic exocrine secretions produced by the intravenous injection of 2-deoxy-D-glucose (a central vagal nerve stimulation), whereas the fragment without a C-terminal amide showed no effect on pancreas. These results indicate that the C-terminal amide of this peptide is necessary to reveal its biological activity.

【 授权许可】

Unknown